|
KMID : 0525720180230010008
|
|
Journal of Chitin and Chitosan
2018 Volume.23 No. 1 p.8 ~ p.14
|
|
|
Improvement of the Quality and Shelf-life of Corn Tortillas by Water Soluble Chitosan
|
|
Loredo-Trevino Araceli
Ocampo-Ramirez Alfonso
Charles-Rodriguez Ana Veronica
Reyes-Vega Maria de la Lu
Aguilar Cristobal N.
Contreras-Esquivel Juan Carlos
|
|
|
Abstract
|
|
|
|
Chitosan-oligosaccharides and water soluble chitosan have wide applications in several industries such as agronomic, cosmetic, feed, food, medicine, and nanotechnology. The aim of this work was to depolymerize chitosan enzymatically using endo-chitosanase from Bacillus sp. and to evaluate the hydrolyzates as fungistatic on corn tortillas prepared with these products. Five water soluble chitosan (2, 4, 8, 12 and 24 h) series were prepared with endo-chitosanase (40ºC, 3.5 U/g chitosan). Two filamentous fungi of contaminated corn tortillas were isolated and later used for the inhibition studies of water soluble chitosan series on agar plates. Furthermore, corn tortillas were elaborated with water soluble chitosan obtained after 4 h of enzyme degradation to evaluate the microbial inhibition effect and sensorial analysis. All water soluble chitosan series were totally soluble in water, except that material modified enzymatically by 2 h. The fungal strains isolated from corn tortillas were Aspergillus sp. and Penicillum sp. Results on microbial radial growth showed that the water soluble chitosan series obtained at the time of 4 h had the biggest effectiveness for the inhibition of growth of both filamentous fungi on agar plate. When the corn tortillas were prepared with water soluble chitosan at a 1% (w/v), a partial inhibition of growth of filamentous fungi was achieved in contrast to the prepared tortillas without this hydrolyzate. Unpleasant flavor was not presented in the corn tortillas by effect of the hydrolyzate addition; this indicates that this material can be used appropriately as food additive in the tortilla industry as inhibitor of microbial growth. This water soluble chitosan can also be considered as a dietary fiber and a prebiotic.
|
|
|
KEYWORD
|
|
|
Fungi, Aspergillus sp., Penicillum sp., Endo-chitosanase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|